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Probiotics may offer benefits beyond gut health. Their viability and colonization in the intestines can be significantly strengthened depending on manufacturing and fermentation conditions, enabling them to provide greater efficacy.

In this study, L. casei HY2782 was compared with a standard strain to evaluate changes in gastrointestinal stability, intestinal cell adhesion, and the expression of genes associated with intestinal barrier function according to fermentation time during milk fermentation.

The results demonstrated the following functional properties of HY2782:

• Superior gastrointestinal viability: Maintained significantly higher survival rates than the standard strain in simulated gastrointestinal digestion conditions
• Significantly improved intestinal cell adhesion: After 7 days of long-term fermentation, adhesion to intestinal cells (HT-29) increased approximately 2.6-fold (3.3% → 8.7%), improving colonization potential
• Strengthened tight junctions: Strengthened the intestinal barrier by significantly increasing the gene expression of tight junction proteins that regulate intestinal permeability
• Maximized Properties with fermentation: Demonstrated that long-term fermentation is an effective manufacturing method to enhance the probiotic properties of HY2782
• Gut health protection mechanism: Contributed to maintaining intestinal barrier integrity based on high viability and adhesion capacity

Analysis based on cell experiments demonstrated that HY2782 can survive longer, adhere better in the gut, and support intestinal barrier gene expression with specific fermentation processes, thereby fundamentally protecting gut health.

Probiotics are not only beneficial for gut health but may also help reduce toxicity caused by fine dust and support overall vitality.

This study investigated the effects of L. casei  HY2782 on the physiological indicators of human intestinal epithelial cells (CCD-18Co) and the model organism C. elegans exposed to fine dust (PM10).

The results demonstrated the following functional properties of HY2782:

• Protection against intestinal cell death: Effectively inhibited apoptosis of human intestinal cells caused by fine dust (cell study)
• Alleviation of oxidative stress and membrane damage: Reduced intracellular reactive oxygen species (ROS) production and prevented fine dust-induced cell membrane disruption (cell study)
• Restoration of reproductive function: Significantly restored egg-laying capacity that had decreased dramatically due to fine dust exposure (animal study)
• Maintenance of motility: Actively restored the movement (body bending) of nematodes impaired by toxic substances (animal study)
• Protection against environmental hazards: Confirmed to act as a strong protective barrier against cellular and physiological toxicity caused by fine dust

Analyses using cells and the model organism demonstrated that HY2782 can contribute to protecting gut health from fine dust, a major health threat in modern life, and preserving overall physiological functions.

Probiotics are not only beneficial for gut health but may also help reduce lung inflammation caused by inhalation of fine dust and strengthen respiratory defense.

This study investigated the changes in lung inflammation markers and antioxidant responses when L. casei HY2782 and B. lactis  HY8002 were orally administered to a mouse model chronically exposed to particulate matter 2.5 (PM2.5).

The results demonstrated the following functional properties of HY2782 and HY8002:

• Inhibition of inflammatory cell infiltration: Effectively prevented the infiltration of eosinophils and neutrophils that exacerbate inflammation in bronchoalveolar lavage fluid (BALF)
• Suppression of leukocyte activation and inflammatory cytokines: Reduced the migration of activated white blood cells and decreased the production of inflammation-inducing substances
• Increased antioxidant enzyme activity: Reduced oxidative stress by increasing the activity of the antioxidant enzymes superoxide dismutase (SOD) and catalase in the blood
• Removal of reactive oxygen species (ROS): Effectively eliminated ROS induced by fine dust, and protected against cellular damage
• Confirmation of respiratory protection mechanism: Protected respiratory health by suppressing lung inflammatory responses caused by fine dust exposure from various angles

Analysis using animal models demonstrated that probiotics HY2782 and HY8002 can act as powerful protective agents against lung damage and inflammation caused by fine dust.

Probiotics are not only beneficial for gut health but may also help alleviate respiratory hypersensitivity such as asthma by regulating allergic immune responses.

This study investigated the changes in respiratory hypersensitivity and inflammatory markers when an L. casei HY2782 complex (including kudzu root extract) was orally administered to a mouse model with allergic asthma induced by egg yolk protein (OVA).

The results demonstrated the following functional properties of the HY2782 complex:

• Inhibition of allergy markers (IgE): Significantly lowered total immunoglobulin E (IgE) levels in serum and bronchoalveolar lavage fluid (BALF)
• Reduction of allergic cytokines (Th2): Effectively regulated the levels of Th2 cytokines, which are major immune factors secreted when allergic reactions are heightened
• Reduction of inflammatory mediators: Reduced the concentrations of various mediators (MCP-1, MIP-2, CXCL-9) that induce lung inflammation
• Prevention of respiratory inflammatory cell influx: Reduced the number of eosinophils and neutrophils in the bronchi and inhibited the infiltration of inflammatory cells into lung tissue (histologically confirmed)
• Synergistic antioxidant and anti-inflammatory effects: The combined antioxidant activity of HY2782 and anti-inflammatory effects of Pueraria root extract comprehensively improved airway hyperresponsiveness

Analysis using animal models demonstrated that the intake of the HY2782 complex can help regulate excessive allergic immune responses, reduce airway sensitivity, and play a positive role in managing respiratory diseases such as asthma.

Probiotics are not only beneficial for gut health but may also help prevent respiratory inflammation caused by ultrafine dust and restore balance in the immune system.

In this study, the efficacy of a complex of **L. casei HY2782 and Pueraria root extract (HY2782 complex)** was analyzed in a mouse model in which respiratory hypersensitivity and inflammation were induced by exposure to particulate matter 2.5 (PM2.5).

The results demonstrated the following functional properties of the HY2782 complex:

• Regulation of immune imbalance cytokines: Significantly reduced Th2-family (IL-4, IL-5, IL-13) and Th17-family (IL-17A) cytokines, which are major factors causing allergies and asthma.
• Suppression of allergy mediators: Soothed hypersensitivity reactions by lowering levels of immunoglobulin E (IgE) and the inflammation-inducing substance leukotriene C4 (LTC4).
• Protection against oxidative stress: Prevented lung cell damage by regulating the expression of genes related to oxidative stress in the body caused by particulate matter
• Inhibition of inflammatory cell infiltration: Alleviated respiratory irritation by significantly reducing the infiltration of neutrophils and eosinophils in bronchoalveolar lavage fluid (BALF).
• Protection of lung tissue: Histological observations confirmed the effective prevention of inflammatory cell infiltration into airway and surrounding lung tissues

Analysis using animal models demonstrated that the HY2782 complex has excellent potential as a material for preventing and managing allergic diseases and respiratory inflammation caused by ultrafine dust inhalation.

It inhibits the production of reactive oxygen species (ROS) caused by fine dust and ultraviolet rays. It improves skin hydration by increasing hyaluronic acid synthase (HAS2) production and reducing hyaluronidases (HYAL1) in skin cells.

It protects skin cells by reducing the expression of oxidative stress markers heme oxygenase 1 (HMOX1) and ferritin light chain (FTL), which become elevated due to fine dust.

It maintains immune homeostasis by regulating (reducing) the expression of IL-37 to suppress excessive immune responses in cells exposed to fine dust.

A complex of HY2782 and Pueraria root extract helps prevent and alleviate respiratory diseases caused by fine dust by inhibiting the excessive secretion of respiratory mucus proteins MUC4, MUC5ac, and MUC16.

It improves colon function and promotes the growth of beneficial bacteria (e.g., Bifidobacterium) with a mixture of probiotics including HY2782 with natural substances such as lactulose and burdock.

Fortified turnip extract is fermented with HY2782 to increase Helicobacter pylori inhibitory activity.

This is a technology that enhances the intestinal barrier function by improving the intestinal cell adhesion ability of the strain through long-term culture and increasing the expression of tight junctions (ZO-1, OCLN, etc.) in intestinal epithelial cells.

HY2782 is used in combination with HY7712 and HY8002 to restore the disrupted intestinal flora in cancer or post-operative patients.

A mixture of HY2782 and Lactobacillus plantarum HY7715 culture increases the expression of tight junctions (ZO-1, TJP-2, Occludin) and mucins (MUC2, MUC4) in intestinal epithelial cells, thereby strengthening intestinal barrier function and alleviating inflammatory bowel disease.

It inhibits neuronal cell death in the brain caused by stress hormones (cortisol/corticosterone) and oxidative stress. By increasing brain-derived neurotrophic factor (BDNF) and cell survival factor (RELA) while lowering the apoptosis factor (caspase-3), it is effective in the treatment and prevention of dementia and depression.