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Probiotics may not only benefit gut health but also aid in weight management by modulating the gut microbial ecosystem to reduce body weight and visceral fat.

This study examined the changes in physical indicators and gut microbiota in 72 overweight adults following 12 weeks of consumption of an L. curvatus HY7601 and L. plantarum KY1032 complex. (Randomized, double-blind, placebo-controlled clinical trial)

The results of probiotic complex (HY7601+KY1032) intake are as follows:

• Significant reduction in weight and waist circumference
• Marked reduction in visceral fat area
• Increase in levels of adiponectin, the "good hormone" that helps burn fat
• Increase in the proportion of beneficial bacteria (Bifidobacteria, Akkermansia, etc.)
• Decrease in the proportion of specific harmful bacteria (Prevotella, etc.) associated with obesity

In overweight adults, 12 weeks of daily intake (1 x 10¹⁰ CFU) showed significant effects in effectively managing body weight and visceral fat by beneficially modulating the gut microbiome.

Probiotics are not only beneficial for gut health but may also be effective in inhibiting fat accumulation and improving metabolism and inflammatory markers in the liver and adipose tissue.

In this study, a high-fat diet-induced obese mouse model was used to evaluate the effects of administering a combination of L. curvatus HY7601 and L. plantarum KY1032 for 10 weeks on body weight and gene expression in liver and adipose tissues.

The results demonstrated the following functional properties of **the probiotic complex (HY7601+KY1032)**:

• Significant reduction in weight gain and body fat accumulation
• Lowering of blood insulin, leptin, cholesterol, and liver toxicity markers
• Normalization of gut microbiota composition altered by obesity
• Suppressed expression of inflammation-inducing genes (TNF, IL-6, etc.) in adipose tissue
• Activation of genes (PGC1, CPT1, etc.) related to lipid oxidation (burning) in liver tissue

After 10 weeks of administration in obese mice, it effectively suppressed obesity by regulating gut microbiota, promoting fat burning in the liver, and reducing inflammation in adipose tissue.

Probiotics are not only beneficial for gut health but may also be effective in improving indicators of metabolic syndrome caused by excessive sugar (fructose) intake.

This study investigated changes in blood and liver tissue markers when a complex of L. curvatus HY7601 and L. plantarum KY1032 was administered for 3 weeks to a mouse model of metabolic syndrome induced by a high-fructose diet (70%).

The results demonstrated the following functional properties of **probiotic complex (HY7601+KY1032)**:

• Significant decrease in blood glucose and insulin levels
• Improvement in triglyceride levels and oxidative stress markers
• Reduction in liver weight and intrahepatic cholesterol levels (at high doses)
• Suppressed expression of lipid synthesis genes (SREBP1, FAS, etc.)
• Activation of fat burning (β-oxidation) genes (PPARα, CPT2)

A 3-week observation in a mouse model of metabolic syndrome demonstrated that the probiotic complex inhibits hepatic fat accumulation and promotes the breakdown of stored fat, and may be a natural alternative for alleviating diet-induced metabolic diseases.

Probiotics are not only beneficial for gut health but may also help with vascular health by lowering blood triglyceride levels and improving lipid metabolism.

This study investigated changes in lipid-related markers in 128 non-diabetic adults with high blood triglyceride levels (hypertriglyceridemia) following 12 weeks of consumption of an L. curvatus HY7601 and L. plantarum KY1032 complex. (Randomized, double-blind, placebo-controlled clinical trial)

The results of probiotic complex (HY7601+KY1032) intake are as follows:

• An 18.3% decrease in blood triglyceride (TG) levels
• A 21.1% increase in apolipoprotein AV (apo AV) levels, which aid in fat breakdown
• A 15.6% increase in LDL cholesterol particle size (larger particles are less harmful to vascular health)
• Effectiveness in all subjects regardless of specific genotype (APOA5).
• More pronounced improvement in the group with higher baseline triglycerides

In adults with hypertriglyceridemia, 12 weeks of daily intake (2 g/day) significantly reduced triglyceride levels and improved lipid metabolism markers, demonstrating beneficial effects for vascular health management.

Probiotics are not only beneficial for gut health but may also be effective in lowering triglyceride levels in the blood and regulating metabolites related to fat metabolism.

In this study, we conducted a detailed analysis of the changes in plasma metabolites and lipid levels in 92 non-diabetic adults with high triglyceride levels following 12 weeks of consumption of an L. curvatus HY7601 and L. plantarum KY1032 complex.

The results of probiotic complex (HY7601+KY1032) intake are as follows:

• A 20% reduction in serum triglyceride (TG) levels
• A 25% increase in apolipoprotein AV (apoA-V) that activates lipid breakdown
• Reduction of 11 types of plasma metabolites, including fatty acid amides (palmitoylamide, etc.) and lysophosphatidylcholine (lysoPC).
• Improvement of fatty acid metabolism efficiency and alleviation of lipid-related inflammatory markers
• Confirmation of a significant correlation between lipid breakdown pathways and metabolite changes in the blood

In adults with high triglyceride levels, 12 weeks of daily intake (1 x 10¹⁰ CFU) significantly reduced triglyceride levels by upregulating the lipid-degrading protein apoA-V and favorably modulating blood metabolites.

Probiotics are not only beneficial for gut health but may also be effective in inhibiting the formation of fat cells and interfering with fat synthesis in liver cells.

This study investigated the effects of a complex of L. curvatus HY7601 and L. plantarum KY1032 (1:1 ratio) on fat accumulation in adipocytes (3T3-L1) and hepatocytes (HepG2).

The results demonstrated the following functional properties of **(HY7601+KY1032) probiotic complex**:

• Significant reduction in lipid accumulation in fat cells (verified by Oil Red O staining)
• Suppression of adipogenesis regulators (PPAR-γ, C/EBP-α) and leptin secretion
• Suppression of mitochondrial production and transcription factors involved in fat synthesis
• Inhibition of the expression of genes related to fatty acid synthesis in liver cells
• Identified that the "cell membrane components" of probiotics play a key role in inhibiting fat formation

Experiments conducted on adipocytes and hepatocytes demonstrated that the probiotic complex is a promising candidate for the prevention and treatment of obesity with the fundamental inhibition of adipogenesis.

Probiotics are not only beneficial for gut health but may also help regulate fat metabolism and generate heat in the body, which can be effective in preventing obesity and arteriosclerosis.

This study investigated changes in body weight and lipid metabolism markers following 7 weeks of administration of an L. curvatus HY7601 and L. plantarum KY1032 complex (1:1 ratio) in a mouse model of obesity induced by a high-fat diet.

The results demonstrated the following functional properties of **(HY7601+KY1032) probiotic complex**:

• Significant reduction in body weight and adipose tissue (white fat, brown fat, etc.) weight
• Concentration-dependent reduction of total blood cholesterol and triglyceride levels
• Promotion of thermogenesis through upregulated expression of the fat-burning protein UCP1
• Activation of cholesterol excretion genes (LXR-α, ABCG5/8, etc.) in the liver and small intestine
• Confirmation of dual metabolic regulation that prevents fat accumulation and aids in excretion and burning

Seven weeks of daily administration (1x10⁹ CFU/kg) improved lipid metabolism and increased energy expenditure (thermogenesis), demonstrating significant effects in weight control as well as the prevention of vascular diseases such as atherosclerosis.

Probiotics are not only beneficial for gut health but may also be effective in inhibiting the maturation of fat cells and preventing the accumulation of body fat.

In this study, we observed how **L. plantarum  KY1032-derived extract (KY1032-CE)** affects lipid accumulation during the process in which adipocyte progenitor cells (3T3-L1) transform into mature adipocytes.

The results demonstrated the following functional properties of the KY1032-derived extract:

• Concentration-dependent reduction of lipid accumulation within fat cells (AdipoRed analysis)
• Marked decrease in the number of mature fat cells containing fat (Oil Red O staining)
• Suppressed expression of key adipogenesis regulators (PPAR-γ, C/EBP-α)
• Reduced expression of fatty acid synthase (FAS) and fat-binding protein (aP2)
• Direct regulation of the process of preadipocytes maturing into adipocytes (adipogenesis).

Cell-based experiments demonstrated the potential of the KY1032-derived extract to contribute to body fat reduction by fundamentally inhibiting adipocyte formation and lipid accumulation.

This invention has a mechanism for lowering blood cholesterol levels by upregulating LDL receptor expression on hepatocyte cell membranes, thereby promoting cellular uptake of blood cholesterol. It additionally suppresses weight gain and significantly reduces body fat mass, particularly epididymal fat, in animal models of obesity induced by a high-fat diet, and can be used as a pharmaceutical composition or health functional food for the prevention of hypercholesterolemia and the suppression of obesity.

This invention demonstrates that by effectively downregulating the mRNA and protein expression of PPARγ2, C/EBPα, FAS, and FABP4, genes that play a key role in the differentiation of preadipocytes (3T3-L1) into mature adipocytes, it inhibits adipocyte differentiation, thereby having the efficacy to fundamentally prevent or ameliorate obesity. It can thus be used as an active ingredient in health functional foods or pharmaceutical compositions for body fat reduction and obesity suppression.