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Probiotics are not only beneficial for gut health but may also be effective for overall anti-aging, such as improving skin elasticity and reducing wrinkle depth, particularly in middle-aged women.

This study examined the effects of 12-week intake of L. plantarum  HY7714 on skin aging indicators in 110 women aged 41 to 59 with dry skin and wrinkles. (Randomized, double-blind, placebo-controlled clinical trial)

The results demonstrated the following functional properties of HY7714:

• Significant increase in skin moisture content in the face and hands
• Inhibition of transepidermal water loss (TEWL) in the face and forearms
• Significant reduction in the depth of wrinkles around the eyes
• Significant improvement in skin radiance
• Significant improvement in skin elasticity (13.17% improvement after 4 weeks, 21.73% improvement after 12 weeks)

In women aged 41–59, daily intake (1 × 10¹⁰ CFU) for 12 weeks improved multiple skin parameters, including hydration, elasticity, and wrinkles, demonstrating its efficacy as a “nutricosmetic” material in a clinical trial.

Probiotics are not only beneficial for gut health but may also be effective in preventing skin dryness caused by UV rays and maintaining skin moisture.

This study investigated the effects of L. plantarum  HY7714 on moisture retention and barrier function in human skin cells (Hs68) and a hairless mouse model.

The results demonstrated the following functional properties of HY7714:

• Increased expression of ceramide synthase (SPT) (cell study)
• Decreased expression of ceramide-degrading enzyme (ceramidase) (cell study)
• Reduction of UVB-induced epidermal thickening (animal study)
• Inhibition of transepidermal water loss (TEWL) and maintenance of hydration (animal study)
• Significant increase in ceramide levels in the skin (animal study)
• Recovery of moisture-related gene damage caused by UV rays (animal study)

When administered daily (1x10⁹ CFU) to hairless mice for 8 weeks, it showed significant effectiveness in regulating ceramide synthesis even under UV exposure conditions, thereby protecting skin barrier function and maintaining moisture levels.

Probiotics are not only beneficial for gut health but may also be effective in preventing UV-induced skin aging (photoaging) and improving wrinkles.

This study investigated the effects of L. plantarum HY7714 on human skin cells and hairless mouse models exposed to ultraviolet (UVB) radiation.

The results demonstrated the following functional properties of HY7714:

• Restoration of collagen production through the inhibition of collagen-degrading enzyme (MMP-1) (cell study)
• Marked reduction in the number, depth, and area of skin wrinkles (animal study)
• Reduction of abnormally thickened epidermal layer caused by UV exposure (animal study)
• Suppression of collagen-degrading enzyme (MMP-13, MMP-2, MMP-9) activity (animal study)
• Effective inhibition of aging-inducing signaling pathways (JNK/c-Jun) (cell study)

Studies using cell and animal models have demonstrated potent anti-aging effects, including protection against UV-induced collagen degradation and direct inhibition of wrinkle formation.

Probiotics are not only beneficial for gut health but may also strengthen the intestinal barrier, suppress inflammation, and improve overall bodily functions.

This study investigated how L. plantarum  HY7714 strengthens the tight junctions (TJs) of intestinal epithelial cells (Caco-2) and affects the breakdown of the intestinal barrier and inflammatory responses.

The results demonstrated the following functional properties of HY7714:

• Restoration of tight junctions (ZO-1, Occludin, Claudin-1): Replenished barrier proteins reduced by inflammation-inducing factors (TNF-α) to strengthen barrier integrity
• Suppressed the production of inflammatory substances (IL-6, IL-8, IL-1β): Significantly lowered the levels of cytokines that trigger inflammatory responses in the gut
• Regulation of barrier-disrupting signaling pathways (NF-κB, MLCK): Helped maintain gut health by suppressing the overexpression of genes that loosen the barrier
• Protection against increased permeability: Maintained strong tight junctions to prevent the entry of harmful external substances

Based on 24-hour observations in intestinal cells, HY7714 was shown to strengthen the intestinal barrier, help protect against inflammation, and establish a foundational gut environment that supports skin health from a “gut-skin axis” perspective.

Probiotics are not only beneficial for gut health but may also be effective in fundamentally improving skin health by regulating the intestinal environment and immune responses.

In this study, we analyzed how the intake of L. plantarum  HY7714 causes changes in the gut microbiome, intestinal barrier function, and related skin health indicators.

The results demonstrated the following functional properties of HY7714:

• Optimization of the gut microbiome: Created a healthy gut ecosystem by increasing beneficial bacteria (Bifidobacterium, etc.) and decreasing harmful bacteria (Proteobacteria) (clinical trial)
• Reduction of intestinal and skin permeability markers and inflammation: Improved the levels of markers related to intestinal and skin permeability (zonulin, calprotectin) and inflammation-related enzymes (MMP-2, MMP-9) (clinical trial)
• Restoration of intestinal barrier integrity: Strengthened the adhesion of compromised barriers by modulating extracellular matrix and immune-related gene expression (cell study)
• Normalization of immune signaling: Activation of gene families required for barrier protection in response to an inflammation-inducing factor (TNF-α) (cell study)

These results demonstrate that HY7714 intake helps regulate the gut environment and reinforce the intestinal barrier, thereby reducing inflammatory factors in the blood and supporting skin health with the “gut–skin axis” mechanism.

Probiotics are not only beneficial for gut health but may also be effective in improving intestinal barrier integrity and protecting against UV-induced skin damage.

This study investigated how exopolysaccharide (EPS) produced by **L. plantarum HY7714** affects the binding capacity of intestinal epithelial cells (Caco-2) and the moisture retention capacity of human skin cells (HS68).

The results demonstrated the following functional properties of HY7714-derived EPS:

• Inducted expression of tight junction gene (OCL-1, ZO-1): Strengthened connections between intestinal cells, thereby enhancing barrier function (intestinal cell study)
• Mitigation of UVB-induced cytotoxicity: Protected skin cells against UV-induced damage (skin cell study)
• Improvement in skin moisture retention: Increased moisture retention even with UV exposure (skin cell study)
• Suppression of the production of reactive oxygen species (ROS) and collagen-degrading enzymes (MMPs): Inhibited components that promote skin aging (skin cell study)
• Demonstration of the functional connection of the gut-skin axis: Demonstrated the mechanism by which substances involved in improving gut health contribute to skin anti-aging

Analysis based on cell experiments demonstrated that EPS produced by HY7714 probiotic is an effective anti-aging substance that not only strengthens the skin barrier but also prevents UV-induced damage and helps maintain skin moisture.

Probiotics are not only beneficial for gut health but may also be effective in reducing intestinal inflammation and protecting against skin damage through the extracellular vesicles (EV) they secrete.

In this study, advanced **"gut-on-a-chip"** technology and cell models were used to analyze the effects of extracellular vesicles (EV) derived from **L. plantarum  HY7714** on intestinal barrier damage and skin cells exposed to UVB.

The results demonstrated the following functional properties of **HY7714-derived EVs**:

• Restoration of intestinal barrier integrity and structural improvement: Repaired the structure of damaged intestinal cells and firmly rebuilt tight junctions (cell study)
• Inhibition of inflammatory pathways (NF-κB, etc.): Significantly suppressed the production of inflammatory cytokines by modulating major inflammatory signaling pathways (cell study)
• Regulation of skin-related proteins: Regulated the expression of proteins involved in skin health and extracellular matrix interactions (e.g., VOC family) (cell study)
• Reduction of UVB-induced skin toxicity: Lowered toxicity in skin cells exposed to UV rays and provided protection (skin cell study)
• Suppression of collagen-degrading enzymes (MMPs): Demonstrated anti-aging potential by reducing the expression of enzymes that promote skin aging (skin cell study)

The results of gut-on-a-chip and cell experiments demonstrated that HY7714-derived EVs not only strengthen the intestinal barrier and control inflammation but also act as key messengers in the "gut-skin axis" to help prevent UV-induced skin aging.

This invention relates to a novel strain, Lactobacillus plantarum  HY7714, which improves skin wrinkles by restoring the production of procollagen in skin cells reduced by ultraviolet (UV) radiation and inhibiting the overexpression of the collagen-degrading enzyme matrix metalloproteinase-1 (MMP-1) induced by UV radiation. Animal studies have shown that this strain restores the thickness of the skin epidermis thickened by UV-induced photoaging, suppresses the expression of MMP-13, increases skin moisture content, and prevents transepidermal water loss (TEWL) to provide a moisturizing effect. These findings support its application as an active ingredient in pharmaceutical compositions, health functional foods, and cosmetic formulations for improving skin wrinkles and enhancing skin hydration.